- Abraham Lincoln Birthplace National Historic Site
- Aleutian World War II National Historic Area (affiliated area)
- Allegheny Portage Railroad National Historic Site
- Andersonville National Historic Site
- Andrew Johnson National Historic Site
- Bent's Old Fort National Historic Site
- Boston African American National Historic Site
- Brown v. Board of Education National Historic Site
- Historic Camden Revolutionary War Site (affiliated area)
- Carl Sandburg Home National Historic Site
- Carter G. Woodson Home National Historic Site
- Charles Pinckney National Historic Site
- Chicago Portage National Historic Site (affiliated area)
- Chimney Rock National Historic Site (affiliated area)
- Christiansted National Historic Site
- Clara Barton National Historic Site
- Edgar Allan Poe National Historic Site
- Edison National Historic Site
- Eisenhower National Historic Site
- Eleanor Roosevelt National Historic Site
- Eugene O'Neill National Historic Site
- First Ladies National Historic Site
- Ford's Theatre National Historic Site
- Fort Bowie National Historic Site
- Fort Davis National Historic Site
- Fort Laramie National Historic Site
- Fort Larned National Historic Site
- Fort Point National Historic Site
- Fort Raleigh National Historic Site
- Fort Scott National Historic Site
- Fort Smith National Historic Site
- Fort Union Trading Post National Historic Site
- Fort Vancouver National Historic Site
- Frederick Douglass National Historic Site
- Frederick Law Olmsted National Historic Site
- Friendship Hill National Historic Site
- Gloria Dei (Old Swedes') Church National Historic Site (affiliated area)
- Golden Spike National Historic Site
- Grant-Kohrs Ranch National Historic Site
- Hampton National Historic Site
- Harry S Truman National Historic Site
- Herbert Hoover National Historic Site
- Home of Franklin D. Roosevelt National Historic Site
- Hopewell Furnace National Historic Site
- Hubbell Trading Post National Historic Site
- James A. Garfield National Historic Site
- Jamestown National Historic Site (affiliated area)
- Jimmy Carter National Historic Site
- John Fitzgerald Kennedy National Historic Site
- John Muir National Historic Site
- Knife River Indian Villages National Historic Site
- Lincoln Home National Historic Site
- Little Rock Central High School National Historic Site
- Longfellow National Historic Site
- Lower East Side Tenement National Historic Site (affiliated area)
- McLoughlin House National Historic Site (affiliated area)
- Maggie L. Walker National Historic Site
- Manzanar National Historic Site
- Martin Luther King, Jr. National Historic Site
- Martin Van Buren National Historic Site
- Mary McLeod Bethune Council House National Historic Site
- Minuteman Missile National Historic Site
- Nicodemus National Historic Site
- Ninety Six National Historic Site
- Palo Alto Battlefield National Historic Site
- Pennsylvania Avenue National Historic Site
- Puukohola Heiau National Historic Site
- Sagamore Hill National Historic Site
- Saint Croix Island International Historic Site
- Saint Paul's Church National Historic Site
- Saint-Gaudens National Historic Site
- Salem Maritime National Historic Site
- San Juan National Historic Site
- Saugus Iron Works National Historic Site
- Sewall-Belmont House National Historic Site (affiliated area)
- Springfield Armory National Historic Site
- Steamtown National Historic Site
- Theodore Roosevelt Birthplace National Historic Site
- Theodore Roosevelt Inaugural National Historic Site
- Thomas Cole National Historic Site (affiliated area)
- Thomas Stone National Historic Site
- Touro Synagogue National Historic Site (affiliated area)
- Tuskegee Airmen National Historic Site
- Tuskegee Institute National Historic Site
- Ulysses S. Grant National Historic Site
- Vanderbilt Mansion National Historic Site
- Washita Battlefield National Historic Site
- Weir Farm National Historic Site
- Whitman Mission National Historic Site
- William Howard Taft National Historic Site
